Dna extraction lab procedure

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DNA is extracted from human cells for a variety of reasons. With a pure sample of DNA you can test anewborn for a genetic disease, analyze forensic evidence, or study a gene involved in cancer. Try thisvirtual laboratory to perform a cheek swab and extract DNA from human cells. Supported by a Science Education Partnership Award (SEPA) Grant No. R25RR023288 from the National Center for Research Resources.The contents provided here are solely the responsibility of the authors and do not necessarily represent the official views of NIH.

First, you need to find something that contains DNA. Since DNA is the blueprint for life, everything living contains DNA.For this experiment, we like to use green split peas. Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).Add 2 tablespoons liquid detergent (about 30ml) and swirl to mix.Let the mixture sit for 5-10 minutes.Pour the mixture into test tubes or other smExtract DNA from Anything LivingIntroduction:Since DNA is theblueprint for life, everything living contains DNA.

DNA isolation is one of the most basic and essential techniquesin the study of DNA. The extraction of DNA from cells and its purification areof primary importance to the field of biotechnology and forensics. Extractionand purification of DNA are the first steps in the analysis and manipulation ofDNA that allow scientists to detect genetic disorders, produce DNA fingerprintsof individuals, and even create genetically engineered organisms that canproduce beneficial products such as insulin, antibiotics, andhormones.DNA can be extractedfrom many types of cells.

The first step is to lyse or break open thecell. This can be done by grinding a piece of tissue in a blender. After thecells have broken open, a salt solution such as NaCl and a detergent solutioncontaining the compound SDActivity 1 - DNA ExtractionWe will extract DNA from fruit to investigate how it looks and feels. This procedure is similar to what scientists have to do before they can use the information contained in this DNA.

However, this is not true. Below, we have outlined a simple experiment showing how DNA can be extracted from a strawberry and made visible to the naked eye. To download a pdf of the information below, please click on the link to your right. Strawberry DNA ExtractionAll living things have DNA: the chemical instructions on how to make a living thing. It can easily be seen with the naked eye when collected from thousands of cells. Carboni, January 2007Text editing by Donald Desaulniers, Ph.D.CONTENTSINTRODUCTION PROCEDURE Summary of the procedure Preliminary operations Preparation of the extracting solution Preparation of the mush Extracting the DNA Filtration Removing the proteins Precipitating the DNA Observation through the microscope CONCLUSION REFERENCESFigure 1: Test tube with DNA extractINTRODUCTIONIn recent years, it is not uncommon to read articles on DNA in bothscientific and popular magazines.

DNA is regularly mentioned in the news and isoften featured in TV detective or crime-scene investigation dramas. DNA, alsoknown as DeoxyriboNucleic Acid, is a long molecule that holds the geneticinformation for all living beings, be it vegetable, animal or a simplemicroorganisms.




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