Extracting dna from your cells lab answers
DNA is extracted from human cells for a variety of reasons. With a pure sample of DNA you can test anewborn for a genetic disease, analyze forensic evidence, or study a gene involved in cancer. Try thisvirtual laboratory to perform a cheek swab and extract DNA from human cells. Supported by a Science Education Partnership Award (SEPA) Grant No. R25RR023288 from the National Center for Research Resources.The contents provided here are solely the responsibility of the authors and do not necessarily represent the official views of NIH.
Vocabulary words:DNA, nucleus, extraction, spooling, What you need:- test tubes with 5ml of detergent solution - capped tubes with 10ml of rubbing alcohol - bottle with prepared salt solution - dixie cups - plastic coffee stirrer - flat wood sticks - - small Eppendorf tube - pipettes Grouping:Students can work in pairs or as individuals. To allow each student to handle materials groups should not be larger than that.
Setting:Classroom or laboratory Time needed:One class period (40- minutes). Students will observe their own DNA. Students willExtract DNA from Anything LivingIntroduction:Since DNA is theblueprint for life, everything living contains DNA. DNA isolation is one of the most basic and essential techniquesin the study of DNA. The extraction of DNA from cells and its purification areof primary importance to the field of biotechnology and forensics.
Extractionand purification of DNA are the first steps in the analysis and manipulation ofDNA that allow scientists to detect genetic disorders, produce DNA fingerprintsof individuals, and even create genetically engineered organisms that canproduce beneficial products such as insulin, antibiotics, andhormones.DNA can be extractedfrom many types of cells.
The first step is to lyse or break open thecell. This can be done by grinding a piece of tissue in a blender. After thecells have broken open, a salt solution such as NaCl and a detergent solutioncontaining the compound SDActivity 1 - DNA ExtractionWe will extract DNA from fruit to investigate how it looks and feels. This procedure is similar to what scientists have to do before they can use the information contained in this DNA.