Extracting dna from fruit
For more thorough analytical work, you need more control over the components of your chemicals, and it may be worth investing in a kit from one of the major suppliers. This is a rough and ready method that should give reasonable quantities of DNA from quite large quantities of material.Lesson organisationYou can run this as a demonstration, or as small group work. Or you could prepare enough of each of several materials to allow groups to take samples from which they extract the DNA.Apparatus and Chemicals.
Extracting DNA from cellsIt is possible to extract DNA from cells in a variety of ways. One of the simplest methods is using peas, soya beans, cress or sweetcorn. Salt water is mixed with detergent and the mashed up substance being tested. The next stage in the method is to filter the mixture and to pour iced ethanol on to the filtrate. Since DNA is the blueprint for life, everything living contains DNA.For this experiment, we like to use green split peas.
Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).Add 2 tablespoons liquid detergent (about 30ml) and swirl to mix.Let the mixture sit for 5-10 minutes.Pour the mixture into test tubes or other smKey conceptsDNAGenomeGenesExtractionLaboratory techniquesIntroductionHave you ever wondered how scientists extract DNA from an organism.
Overall, DNA tells an organism how to develop and function, and is so important that this complex compound is found in virtually every one of its cells. Each cell has an entire copy of the same set of instructions, and this set is called the genome. Think about seesaws (or teeter totters) and how to balance them. For this lab, we are extracting DNA from a Kiwi fruit. This procedure is similar to what scientists have to do before they can use the information contained in this DNA.
This simple experiment will show you how to extract DNA from fruit like a banana or strawberry. All you need are some fruit and some things you probably have around the house right now.What you need. Before you start:A blue print is a detailed drawing or map that directs the construction of a building. DNA (deoxyribonucleic acid) is the hereditary material that directs the construction of living things.
Gently mix until it is a uniform consistency. Filter until there is about 2 tsp oHow to Extract DNA From FruitsHow to Extract DNA From FruitsG. Carboni, January 2007Text editing by Donald Desaulniers, Ph.D.CONTENTSINTRODUCTION PROCEDURE Summary of the procedure Preliminary operations Preparation of the extracting solution Preparation of the mush Extracting the DNA Filtration Removing the proteins Precipitating the DNA Observation through the microscope CONCLUSION REFERENCESFigure 1: Test tube with DNA extractINTRODUCTIONIn recent years, it is not uncommon to read articles on DNA in bothscientific and popular magazines.
DNA is regularly mentioned in the news and isoften featured in TV detective or crime-scene investigation dramas. DNA, alsoknown as DeoxyriboNucleic Acid, is a long molecule that holds the geneticinformation for all living beings, be it vegetable, animal or a simplemicroorganisms. It is capable of copying itself and can synthesize RNA (RiboNucleicAcid).